The Knoxville racer had to serve a pass-thru penalty after speeding on pit road.
The driver of the No. 78 Toyota Camry led a record 388 of 400 laps.
With the win, Tennessee improved their overall mark to 23-27 in the first half.
Rossi did it was a fuel conservation strategy that saw him run the final 36 laps without a pit stop.
With the loss, Tennessee drops to 22-27 on the season.